Mouse Anti-Virus Influenza Virus hemagglutinin Hybridoma [84] (CSC-H1168)

This hybridoma produces mAbs (IgG2a) against virus Influenza virus hemagglutinin Datasheet

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General Information


Immunogen
Purified influenzavirus Bangkok A (1/79)
Isotype
IgG2a
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
84
Cell Line Description
Animals were immunized with purified influenzavirus Bangkok A (1/79). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
DMEM with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% FBS.
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Target


Introduction
Influenza hemagglutinin (HA) or haemagglutinin (British English) is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.

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