House Anti-Virus Influenza A equine 2 Virus Heterohybridoma [BGSD 28E2] (CSC-H0352)

This hybridoma produces mAbs against virus Influenza A equine 2 virus Datasheet

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General Information


Immunogen
Influenza A equine 2 virus
Fusion Species
Horse X Mouse Heterohybridoma
Immunological Donor
Horse peripheral lymphocytes
Myeloma
NS0
Clone
BGSD 28E2
Cell Line Description
The heterohybridoma was produced by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
RPMI 1640 with 2 mM glutamine, 1mM Sodium Pyruvate (NaP), supplemented with 5%(v/v) FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere and maintain cultures between 3-9x10^5 cells/ml.
Storage
Liquid nitrogen vapor phase.

Target


Introduction
Equine influenza (horse flu) is the disease caused by strains of influenza A that are enzootic in horse species. Equine influenza occurs globally, and is caused by two main strains of virus: equine-1 (H7N7) and equine-2 (H3N8). [citation needed] The disease has a nearly 100% infection rate in an unvaccinated horse population with no prior exposure to the virus.

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