Anti-IRF3 monoclonal antibody, clone FQS3529Z Knockout Validated

Antibody Isotype

IgG

Clone

FQS3529Z

Species Reactivity

Mouse; Human

Immunogen

A synthetic peptide corresponding to residues surrounding the N terminus of human IRF3.

Conjugate

Application Notes

ICC/IF: 1/100; WB: 1/1000; IHC-P: 1/500; Flow Cyt: 1/160.

Alternative Names

IRF3; interferon regulatory factor 3;

Entrez Gene ID

UniProt ID

Antigen Description

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Pathway

Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Apoptosis, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem;