Anti-IPO7 polyclonal antibody (DPABH-04608)

Goat anti-Human IPO7 (aa 1026-1038) polyclonal antibody for WB,ELISA,IHC-P Datasheet

Bring this labeled antibody directly to your bench!

Online Inquiry Add to basket

Specifications


Host Species
Goat
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Synthetic peptide: SSFNFGGPAPGMN with a Cysteine residue linker, corresponding to C terminal amino acids 1026 - 1038 of Human Importin 7.
Conjugate
Unconjugated

Applications


Application Notes
WB: 0.1 - 1 μg/ml; ELISA:1/32000; IHC-P: 5 μg/ml.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
IPO7; importin 7; Imp7; RANBP7; importin-7; RAN-binding protein 7
Entrez Gene ID
UniProt ID

Product Background


Gene summary
IPO7 (Importin 7) is a Protein Coding gene. Diseases associated with IPO7 include hiv-1. GO annotations related to this gene include binding and histone binding. An important paralog of this gene is IPO8. The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.
Antigen Description
Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Importin-7 is a protein that in humans is encoded by the IPO7 gene. 0The function about IPO7 antigen include Ran GTPase binding; histone binding; protein binding; small GTPase regulator activity.

Citations


Have you cited DPABH-04608 in a publication? Let us know and earn a reward for your research.

References


Bannister, AJ; Miska, EA; et al. Acetylation of importin-alpha nuclear import factors by CBP/p300. CURRENT BIOLOGY 10:467-470(2000).
Mizuguchi, C; Moriyama, T; et al. Generation and Characterization of a Monoclonal Antibody Against Importin alpha 7/NPI-2. HYBRIDOMA 30:307-309(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !

Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * Please input "diagnostics" as verification code.

Online Inquiry

Order Info: Anti-IPO7 polyclonal antibody

Online Inquiry
  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!
  15% off your first purchase

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket