Mouse Anti-Bovine INS Hybridoma [EC0H9] (CSC-H1252)

This hybridoma produces mAbs (IgG2a, kappa light chain) against bovine INS

General Information


Immunogen
Purified beef insulin
Isotype
IgG2a, kappa light chain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
EC0H9
Cell Line Description
Animals were immunized with purified beef insulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to insulin from human, cow, pig, rabbit, rat and chicken and to pro-insulin from pig and cow. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
DMEM
with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate
, supplemented with 10% FBS.
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.

Target


Synonyms
INS;insulin;proinsulin;
Entrez Gene ID

Citations


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