Mouse Anti-Human INS Hybridoma [DH8D8] (CSC-H1236)

This hybridoma produces mAbs (IgG1, kappa light chain) against human INS

General Information


Immunogen
Zinc containing crystalline human insulin
Isotype
IgG1, kappa light chain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
DH8D8
Cell Line Description
Animals were immunized with zinc containing crystalline human insulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to insulin from human, pig, cow, rabbit and sheep and to pro-insulin from cow and pig. Weaker reactions were found with fish, guinea pig and chicken insulin. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
DMEM with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% FBS.
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.

Target


Synonyms
INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10;
Entrez Gene ID
UniProt ID

Citations


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