Mouse Anti-Human INS Hybridoma [DF0I0] (CSC-H1219)

This hybridoma produces mAbs (IgG1, kappa light chain) against human INS

General Information


Immunogen
Zinc containing crystalline human insulin
Isotype
IgG1, kappa light chain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
DF0I0
Cell Line Description
Animals were immunized with zinc containing crystalline human insulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to insulin from human, cow, pig and rabbit and to pro-insulin from cow and pig. The antibody cross-reacts with insulin from sheep, guinea pig, chicken and fish. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
DMEM with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% FBS.
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Target


Synonyms
INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10;
Entrez Gene ID
UniProt ID

Citations


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