Mouse Anti-Bovine INS Hybridoma [DD0D21] (CSC-H1200)

This hybridoma produces mAbs (IgG1, kappa light chain) against bovine INS

Datasheet

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Summary
Documentation

General Information

Immunogen

Beef insulin

Isotype

IgG1, kappa light chain

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

Sp2/0-Ag14

Clone

DD0D21

Cell Line Description

Animals were immunized with beef insulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds insulin from human, cow, pig, rabbit, rat and sheep. The antibody also binds pro-insulin from chicken, pig and cow. The antibody binds guinea pig, and fish insulins poorly. Tested and found negative for ectromelia virus (mousepox).

Morphology

Lymphoblast

Growth Properties

Suspension

Culture Method

Complete Growth Medium

DMEM with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% FBS.

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.