Mouse Anti-Bovine INS Hybridoma [CF4G0] (CSC-H1183)

This hybridoma produces mAbs (IgG2a, kappa light chain) against bovine INS

Datasheet

COA

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Summary
Documentation

General Information

Immunogen

Beef insulin

Isotype

IgG2a, kappa light chain

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

Sp2/0-Ag14

Clone

CF4G0

Cell Line Description

Animals were immunized with beef insulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to insulins of cow, pig, human, sheep and rabbit. The antibody cross-reacts with insulin from chicken, guinea pig and fish and pro-insulin from pig and cow. Tested and found negative for ectromelia virus (mousepox).

Morphology

Lymphoblast

Growth Properties

Suspension

Culture Method

Complete Growth Medium

DMEM with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% FBS.

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.

Freezing medium: to complete growth medium, add 5%(v/v) DMSO