Anti-IGF2R monoclonal antibody, clone 483727

Name: Anti-IGF2R monoclonal antibody, clone 483727
SKU: DCABY-4169
Rating: 5 (1 reviews)

Mouse Anti-Human IGF2R monoclonal antibody for ELISA(Cap)

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG2b

Clone

483727

Species Reactivity

Human

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGF-II R. Ser1510-Phe2108 Accession Number P11717

Conjugate

Unconjugated

Applications

Application Notes

ELISA Capture: 2-8 μg/mL; ELISA Detection: 0.1-0.4 μg/mL
We recommend the following for sandwich ELISA (Capture - Detection):
DCABY-4169 - DPABY-450

Target

Alternative Names

IGF2R; insulin-like growth factor 2 receptor; MPR1; MPRI; CD222; CIMPR; M6P-R; cation-independent mannose-6-phosphate receptor; M6PR; CI-MPR; MPR 300; M6P/IGF2R; IGF-II receptor; M6P/IGF2 receptor; CI Man-6-P receptor; 300 kDa mannose 6-phosphate receptor

Entrez Gene ID

3482

UniProt ID

P11717

Product Background

Antigen Description

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues.IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment segment and a 124 aa cytoplasmic tail. IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, It would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II fordestruction in lysosomes.The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligandbindingalpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF-I R stimulates intrinsic kinase activity.

Pathway

Clathrin derived vesicle budding; Golgi Associated Vesicle Biogenesis; Lysosome; Membrane Trafficking; trans-Golgi Network Vesicle Budding;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

The mannose 6-phosphate/insulin-like growth factor 2 receptor mediates plasminogen-induced efferocytosis

JOURNAL OF LEUKOCYTE BIOLOGY

Authors: Ohradanova-Repic, Anna; Machacek, Christian; Donner, Clemens; Muehlgrabner, Vanessa; Petrovcikova, Eva; Zahradnikova, Alexandra, Jr.; Vicikova, Kristina; Horejsi, Vaclav; Stockinger, Hannes; Leksa, Vladimir

Abstract

The plasminogen system is harnessed in a wide variety of physiological processes, such as fibrinolysis, cell migration, or efferocytosis; and accordingly, it is essential upon inflammation, tissue remodeling, wound healing, and for homeostatic maintenance in general. Previously, we identified a plasminogen receptor in the mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R, CD222). Here, we demonstrate by means of genetic knockdown, knockout, and rescue approaches combined with functional studies that M6P/IGF2R is up-regulated on the surface of macrophages, recognizes plasminogen exposed on the surface of apoptotic cells, and mediates plasminogen-induced efferocytosis. The level of uptake of plasminogen-coated apoptotic cells inversely correlates with the TNF-alpha production by phagocytes indicating tissue clearance without inflammation by this mechanism. Our results reveal an up-to-now undetermined function of M6P/IGF2R in clearance of apoptotic cells, which is crucial for tissue homeostasis.

The Function of Long Non-coding RNAs in Genomic Imprinting

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

Authors: Yang Wen-Zhi; Zhang Cui; Wang Guan-Nan; Li Dong-Jie; Li Shi-Jie

Abstract

Long noncoding RNAs (lncRNA) are non-protein coding transcripts longer than 200 nucleotides. The field of long noncoding RNA (lncRNA) research has been rapidly advancing in recent years as its large number and important biological functions. Genomic imprinting is an epigenetic phenomenon. The lncRNAs play a critical role in important biological functions by establishing genomic imprinting of target genes. Genomic imprinting has been a great resource for studying transcriptional and post-transcriptional-based gene regulation by lncRNAs. This review will focus on the mechanisms of imprinting in six of the most well-studied imprinted gene clusters: Kcnq1/Cdkn1c, Igf2r/Airn, Prader-Willi (PWS)/Angelman (AS), Snurf/Snrpn, Dlk1-Dio3, H19/Igf2, and overview the functional role of antisense lncRNAs (Kcnq1ot1t1, Airn and Ube3a-ATS), intergenic lncRNAs (H19, IPW and MEG3), and enhancer lncRNAs (IG-DMR eRNAs) to understand the diverse mechanisms being employed by them in cis and (/or) trans to regulate the parent-of-origin-specific expression of target genes. A better understanding of these downstream mechanisms will help to improve our general understanding of the function of ncRNAs throughout the genome.