Anti-IGD monoclonal antibody (CABT-49901MR)

Mouse anti-IR731 IGD monoclonal antibody for IHC-Fr, ELISA.

Additional Formats Available

Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
MARD-3
Species Reactivity
Rat
Immunogen
IR731 myeloma protein
Conjugate
Unconjugated

Target


Alternative Names
Ighd; Immunoglobulin heavy constant delta; IgD; Immunoglobulin D; Immunoglobulin δ; IgD heavy chain
Entrez Gene ID
UniProt ID
P01883

Citations


Have you cited CABT-49901MR in a publication? Let us know and earn a reward for your research.

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


THE USE OF THE POLYMERASE CHAIN-REACTION IN PRENATAL-DIAGNOSIS OF GROWTH-HORMONE GENE DELETIONS

CLINICAL ENDOCRINOLOGY

Authors: MULLIS, PE; BRICKELL, PM

OBJECTIVE Familial isolated growth hormone deficiency (IGHD) type IA is characterized by a complete absence of human growth hormone (hGH) resulting in most cases from either a 6.7 or 7.7 kb deletion of DNA containing the hGH-1 gene. These patients have a strong initial anabolic response to exogenous recombinant hGH (r-hGH) therapy, frequently associated with the development of immune intolerance to r-hGH which causes an arrest of response to r-hGH replacement. This disorder is inherited as an autosomal recessive trait. PATIENTS AND DESIGN In two pregnancies at risk, the polymerase chain reaction (PCR) was applied as a method for identifying hGH-1 gene deletions in DNA obtained by chorionic villus sampling (CVS) in the first trimester. RESULTS Homozygotes for the 6.7kb deletion of DNA containing the hGH-1 gene were easily and conclusively detected by the absence of 1900, 761 and 712bp fragments after Smal digestion of the polymerase chain reaction products. In contrast, the pattern found in heterozygotes for the hGH-1 gene deletion was difficult to distinguish from the pattern found in normal homozygotes. CONCLUSIONS We conclude that the polymerase chain reaction method is valuable for diagnosing individuals who are homozygous for hGH-1 gene deletions, while heterozygotes and normal individuals may be difficult to distinguish from each other. We suggest that, in these cases, Southern blotting remains the analysis to perform.

Retesting young adults with childhood-onset growth hormone (GH) deficiency with GH-releasing-hormone-plus-arginine test

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Authors: Aimaretti, G; Baffoni, C; Bellone, S; Di Vito, L; Corneli, G; Arvat, E; Benso, L; Camanni, F; Ghigo, E

Within an appropriate clinical context, severe GH deficiency (GHD) in adults has to be defined biochemically by provocative testing of GH secretion. Patients with childhood-onset GHD need retesting in late adolescence or young adulthood to verify whether they have to continue recombinant human GH treatment. GHRH + arginine (GHRH + ARG) is the most reliable alternative to the insulin-induced hypoglycemia test (ITT) as a provocative test for the diagnosis of GHD in adulthood, provided that appropriate cut-off limits are assumed (normal limits, 16.5 mu g/L as 3rd and 9.0 mu g/L as Ist centile). We studied the GH response to a single GHRH (1 mu g/kg iv) + ARG (0.5 g/kg iv) test in 62 young patients who had undergone GH replacement in childhood, based on the following diagnosis: 1) organic hypopituitarism with GHD (oGHD) [n = 18: 15 male (M), 3 female (F); age, 26.8 +/- 2.2 yr; GH peak < 10 mu g/L after two classical tests]; 2) idiopathic isolated GHD (iGHD) [n = 23 (15 M, 8 F); age, 23.0 +/- 1.5 yr; GH peak < 10 mu g/L after two classical tests]; and 3) GH neurosecretory dysfunction (GHNSD) [n = 21 (10 M, 11 F); age, 25.1 +/- 1.6 yr; GH peak > 10 mu g/L after classical test but mGHc < 3 mu g/L]. The GH responses to GHRH + ARG in these groups were also compared with that recorded in a group of age-matched normal subjects (NS) [n = 48 (20 M, 28 F); age, 27.7 +/- 0.8 yr]. Insulin-like growth factor I levels in oGHD subjects (61.5 +/- 13.7 mu g/L) were lower (P < 0.001) than those in iGHD subjects (117.2 +/- 13.1 mu g/L); the latter were lower than those in GHNSD subjects (210.2 +/- 12.9 mu g/L),which, in turn, were similar to those in NS (220.9 +/- 7.1 mu g/L). The mean GH peak after GHRH + ARG in oGHD (2.8 +/- 0.8 mu g/L) was lower (P < 0.001) than that in iGHD (18.6 +/- 4.7 mu g/L), which, in turn, was clearly lower (P < 0.001) than that in GHNSD (31.3 +/- 1.6 mu g/L). The GH response in GHNSD was lower than that in NS (65.9 +/- 5.5 mu g/L), but this difference did not attain statistical significance. With respect to the 3rd centile limit of GH response in young adults (i.e. 16.5 mu g/L), retesting confirmed CHD in all oGHD, in 65.2% of iGHD, and in none of the GHNSD subjects. With respect to the Ist centile limit of GH response (i.e. 9.0 mu g/L), retesting demonstrated severe GHD in 94% oGHD and in 52.1% of iGHD. All oGHD and iGHD with GH peak after GHRH + ARG lower than 9 mu g/L had also GH peak lower than 3 mu g/L after ITT. In the patients in whom GHD was confirmed by retesting, the mean GH peak after GHRH + ARG was higher than that after ITT (3.4 +/- 0.5 us. 1.9 +/- 0.4). In conclusion, given appropriate cut-off limits, GHRH + ARG is as reliable as ITT for retesting patients who had undergone GH treatment in childhood. Among these patients, severe GHD in adulthood is generally confirmed in oGHD, is frequent in iGHD, but never occurs in GHNSD.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:
Verification code
Click image to refresh the verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket