Anti-SIRT2 monoclonal antibody (CABT-35791RH)
Rabbit Anti-SIRT2 monoclonal antibody for WB, ICC/IF, IP, FC
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Human, Mouse, Rat
Recommended Dilution: WB: 1:500-1:1,000; ICC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: ICC staining SIRT2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining SIRT2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of Hela cells with SIRT2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody
FLJ35621; FLJ37491; NAD dependent deacetylase sirtuin 2; NAD-dependent deacetylase sirtuin-2; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2
SIRT2 (Sirtuin 2) is a Protein Coding gene. Among its related pathways are p53 Signaling and Signaling events mediated by HDAC Class III. GO annotations related to this gene include chromatin binding and histone deacetylase binding. An important paralog of this gene is SIRT3. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene.
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Imaoka, N; Hiratsuka, M; et al. Prognostic significance of sirtuin 2 protein nuclear localization in glioma: An immunohistochemical study. ONCOLOGY REPORTS 28:923-930(2012).
Zhang, SF; Wu, HW; et al. Gold Nanoparticle Based Fluorescence Resonance Energy Transfer Immunoassay for the Detection of the Histone Deacetylase Activity using a Fluorescent Peptide Probe. ANALYTICAL LETTERS 46:2029-2039(2013).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.