Anti-S100B monoclonal antibody (DMABT-H27120H)
Rabbit Anti-S100B monoclonal antibody for WB, ICC/IF, IP, IHC
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Human, Mouse, Rat, Goat, zebrafish
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of S100 beta on different lysates using anti-S100 beta antibody at 1/1,000 dilution.
Lane 1: Mouse liver;
Lane 2: Mouse heart
Lane 3: Hela
Fig2: ICC staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.
NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S-100 protein beta chain
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Feng, L; Schmidt, AM; et al. Chronic vascular inflammation in patients with type 2 diabetes - Endothelial biopsy and RT-PCR analysis. DIABETES CARE 28:379-384(2005).
Maetzler, W; Apel, A; et al. Comparable Autoantibody Serum Levels against Amyloid- and Inflammation-Associated Proteins in Parkinson's Disease Patients and Controls. PLOS ONE 9:-(2014).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.