Anti-S100B monoclonal antibody (DMABT-H27120H)

Rabbit Anti-S100B monoclonal antibody for WB, ICC/IF, IP, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TD68-13
Species Reactivity
Human, Mouse, Rat, Goat, zebrafish
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of S100 beta on different lysates using anti-S100 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver; Lane 2: Mouse heart Lane 3: Hela
Fig2: ICC staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.

Target


Alternative Names
NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S-100 protein beta chain

Citations


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References


Bastian, A; Negroiu, G; et al. Tyrosinase and TRP2 expression in hipomelanotic malignant melanoma. PROCEEDINGS OF THE 3RD INTERCONTINENTAL CONGRESS OF PATHOLOGY - FREE PAPERS :201-207(2008).
Mu, LL; Zhang, Y; et al. Activation of the receptor for advanced glycation end products (RAGE) exacerbates experimental autoimmune myasthenia gravis symptoms. CLINICAL IMMUNOLOGY 141:36-48(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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