Anti-PTEN monoclonal antibody (CABT-36410RH)

Rabbit Anti-PTEN monoclonal antibody for WB, ICC/IF, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TK20-14
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:100-1:500; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of PTEN on different lysates using anti-PTEN antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7; Lane 2: Hela Lane 3: 293T
Fig2: ICC staining PTEN in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PTEN in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining PTEN in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PTEN antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PTEN antibody. Counter stained with hematoxylin.

Target


Alternative Names
10q23del; BZS; DEC; GLM2; MGC11227; MHAM

Citations


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References


Razis, E; Pentheroudakis, G; et al. EGFR gene gain and PTEN protein expression are favorable prognostic factors in patients with KRAS wild-type metastatic colorectal cancer treated with cetuximab. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 140:737-748(2014).
Tamura, M; Yanagihara, N; et al. Activation of DNA synthesis and AP-1 by profilin, an actin-binding protein, via binding to a cell surface receptor in cultured rat mesangial cells. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 11:1620-1630(2000).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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