Anti-Human MET monoclonal antibody (CABT-35309RH)

Rabbit Anti-Human MET monoclonal antibody for WB, ICC/IF, IHC, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TK20-16
Species Reactivity
Human
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Met on different lysates using anti-Met antibody at 1/1,000 dilution. Positive control: Lane 1: Hela; Lane 2: HepG2
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Met antibody. Counter stained with hematoxylin.
Fig2: ICC staining Met in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Met antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Met antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Met antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with Met antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
AUTS9; c met; D249; Hepatocyte growth factor receptor; HGF; HGF receptor
Entrez Gene ID
UniProt ID

Citations


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References


Kim, JS; Park, HM; et al. A Pepper MSRB2 Gene Confers Drought Tolerance in Rice through the Protection of Chloroplast-Targeted Genes. PLOS ONE 9:-(2014).
Camidge, DR; Pao, W; et al. Acquired resistance to TKIs in solid tumours: learning from lung cancer. NATURE REVIEWS CLINICAL ONCOLOGY 11:473-481(2014).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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