Anti-CENPF monoclonal antibody (CABT-32574MH)

Mouse anti-Human CENPF monoclonal antibody for FC, ICC/IF, IHC-Fr, IHC-P, IP, WB Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Clone
25D20 /2E9
Species Reactivity
Human
Immunogen
Fusion protein, corresponding to amino acids 1759-2093 of Human Mitosin/CENPF.
Conjugate
Unconjugated

Applications


Application Notes
IHC-P: 1/200; Flow Cyt: 0.5μg/106 cells.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
CENPF; centromere protein F, 350/400kDa (mitosin); centromere protein F (350/400kD, mitosin); centromere protein F; hcp 1; AH Antigen
Entrez Gene ID
UniProt ID

Product Background


Gene summary
CENPF (Centromere Protein F) is a Protein Coding gene. Diseases associated with CENPF include ciliary dyskinesia, primary, 31 and lethal fetal brain malformation-duodenal atresia-bilateral renal hypoplasia syndrome. Among its related pathways are Signaling by GPCR and Signaling by Rho GTPases. GO annotations related to this gene include protein homodimerization activity and transcription factor binding. This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
Antigen Description
CCR7, or C-C chemokine receptor type 7, is a G protein-coupled, seven-transmembrane domain receptor protein. Originally identified as the EBV-induced gene-1, CCR7 was later found to be the receptor for the chemokines CCL21 (SLC, 6Ckine, Exodus-2) and CCL1Ciliary dyskinesia, primary, 31 (CILD31) [MIM:616369]: A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia. Patients may exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. Note=The disease is caused by mutations affecting the gene represented in this entry. Centromere protein F is a protein that in humans is encoded by the CENPF gene. 0The function about CENPF antigen include chromatin binding; dynein binding; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding.
Pathway
Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem; Mitotic Prometaphase, organism-specific biosystem.

Citations


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References


Hamdouch, K; Rodriguez, C; et al. Anti-CENPI autoantibodies in scleroderma patients with features of autoimmune liver diseases. CLINICA CHIMICA ACTA 412:2267-2271(2011).
Liu, H; Zhang, JL; et al. Screening of autoantibodies as potential biomarkers for hepatocellular carcinoma by using T7 phase display system. CANCER EPIDEMIOLOGY 36:82-88(2012).

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