House Anti-Virus Haemagglutinin Heterohybridoma [BGSD 95C5] (CSC-H0356)

This hybridoma produces mAbs (IgG) against virus Haemagglutinin Datasheet

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General Information


Immunogen
Influenza A equine 2 virus
Isotype
IgG
Fusion Species
Horse X Mouse/Horse Heterohybridoma
Immunological Donor
Horse sub-mandibular lymph node
Myeloma
AFRC 17D1
Clone
BGSD 95C5
Cell Line Description
The heterohybridoma is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus
Growth Properties
Suspension

Culture Method


Complete Growth Medium
RPMI 1640 with 2 mM glutamine, 1mM Sodium Pyruvate (NaP), supplemented with 5%(v/v) FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere and maintain cultures between 3-9x10^5 cells/ml.
Storage
Liquid nitrogen vapor phase.

Target


Introduction
Hemagglutinin or haemagglutinin (British English) refers to a substance that causes red blood cells to agglutinate. This process is called hemagglutination or haemagglutination. Antibodies and lectins are commonly known hemagglutinins. Hemagglutination can be used to identify RBC surface antigens (with known antibodies) or to screen for antibodies (with RBCs with known surface antigens). Using anti-A and anti-B antibodies that bind specifically to either the A or to the B blood group surface antigens on RBCs it is possible to test a small sample of blood and determine the ABO blood group (or blood type) of an individual. The bedside card method of blood grouping relies on visual agglutination to determine an individuals blood group. The card has dried blood group antibody reagents fixed onto its surface and a drop of the individuals blood is placed on each area on the card. The presence or absence of visual agglutination enables a quick and convenient method of determining the ABO and Rhesus status of the individual. Agglutination of red blood cells is used in the Coombs test.

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