House Anti-Virus Haemagglutinin Heterohybridoma [BGSD 67E3] (CSC-H0353)

This hybridoma produces mAbs (IgG) against virus Haemagglutinin

General Information

Influenza A equine 2 virus
Fusion Species
Horse X Mouse/Horse Heterohybridoma
Immunological Donor
Horse peripheral lymph node
Cell Line Description
The heterohybridoma is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus.
Growth Properties

Culture Method

Complete Growth Medium
RPMI 1640 with 2 mM glutamine, 1mM Sodium Pyruvate (NaP), supplemented with 5%(v/v) FBS
Incubate cells at 37°C with 5% CO2 in air atmosphere and maintain cultures between 3-9x10^5 cells/ml.
Liquid nitrogen vapor phase.


Hemagglutinin or haemagglutinin (British English) refers to a substance that causes red blood cells to agglutinate. This process is called hemagglutination or haemagglutination. Antibodies and lectins are commonly known hemagglutinins. Hemagglutination can be used to identify RBC surface antigens (with known antibodies) or to screen for antibodies (with RBCs with known surface antigens). Using anti-A and anti-B antibodies that bind specifically to either the A or to the B blood group surface antigens on RBCs it is possible to test a small sample of blood and determine the ABO blood group (or blood type) of an individual. The bedside card method of blood grouping relies on visual agglutination to determine an individuals blood group. The card has dried blood group antibody reagents fixed onto its surface and a drop of the individuals blood is placed on each area on the card. The presence or absence of visual agglutination enables a quick and convenient method of determining the ABO and Rhesus status of the individual. Agglutination of red blood cells is used in the Coombs test.


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A Reactive Oxygen Species Scavenging and O-2 Generating Injectable Hydrogel for Myocardial Infarction Treatment In vivo


Authors: Ding, Jie; Yao, Yuejun; Li, Jiawei; Duan, Yiyuan; Nakkala, Jayachandra Reddy; Feng, Xue; Cao, Wangbei; Wang, Yingchao; Hong, Liangjie; Shen, Liyin; Mao, Zhengwei; Zhu, Yang; Gao, Changyou

The excessive reactive oxygen species (ROS) and hypoxia deteriorate the inflammation-related diseases such as myocardial infarction (MI), and thereby deter the normal tissue repair and recovery and further lead to severe fibrosis and malfunction of tissues and organs. In particular, the MI has become one of the leading causes of death nowadays. In this study, a novel type of injectable hydrogel with dual functions of ROS scavenging and O-2 generating is fabricated for MI treatment in vivo. The hydrogel is formed within 3 s from the synthetic ROS-cleavable hyperbranched polymers and methacrylate hyaluronic acid (HA-MA) under UV-irradiation. Addition of biocompatible and applicable catalase in vivo enables the further transition of H2O2, a major type of ROS, to O-2 and H2O. Results of rat MI model demonstrate that this hydrogel can significantly remove excessive ROS, inhibit cell apoptosis, increase M2/M1 macrophage ratio, promote angiogenesis, reduce infarcted area, and improve cardiac functions. With the appropriate degradation rate, simple structure and composition without cell seeding, and very excellent MI therapeutic effect, this ROS scavenging and O-2 generating hydrogel has a great promise to be applied clinically.

Single cell protein production from food waste using purple non-sulfur bacteria shows economically viable protein products have higher environmental impacts


Authors: LaTurner, Zachary W.; Bennett, George N.; San, Ka-Yiu; Stadler, Lauren B.

Landfilling of food waste in the United States is a source of unutilized resources and environmental risks. As cities look to divert food waste from landfills, microorganisms may hold the answer. Purple non-sulfur bacteria are a group of microorganisms that can treat and extract resources from food waste to produce a protein supplement for animal feed. This study includes a life cycle assessment to compare four food waste management scenarios: purple non-sulfur bacteria production offsetting soybean meal production, purple non-sulfur bacteria production offsetting fishmeal and carotenoid production, anaerobic digestion, and landfilling (status quo). Purple non-sulfur bacteria offsetting soybean meal production resulted in significantly reduced environmental impacts compared to landfilling. Specifically, the eutrophication impact, land use, and water use for the soybean meal offset scenario were 141 kg N equivalents/day, 96 ha, and 120,000 L/day lower than landfilling, respectively. However, the low value of soybean meal limits the economic viability of this scenario. When high-value fishmeal and carotenoids were the offset products, there was an increase in value of $0.023/kg FW treated over landfill disposal of food waste. Offset fishmeal and carotenoids are not without environmental tradeoffs, most significantly with respect to eutrophication and global warming. This study then demonstrated that determining maximal growth rates to a PNSB photobioreactor, growing PNSB microorganisms on sunlight, and identifying the degree to which PNSB carotenoids can replace carotenoids in fish food were plausible lines of research to improve environmental and/or economic viability of the process. The life cycle assessment developed here can inform decision making for food waste management and guide research to improve purple non-sulfur bacteria production. (C) 2020 Elsevier Ltd. All rights reserved.

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