Mouse Anti-Human HSP90B1 Monoclonal Antibody (DPABH-01087)

Mouse Anti-Human HSP90B1 Monoclonal Antibody for IHC, WB, ICC-IF Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Species Reactivity
Human
Immunogen
Recombinant Protein, antigen sequence: SGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTA
Conjugate
unconjugated

Applications


Application Notes
ICC-IF: 1-4 µg/ml
IHC: 1:500 - 1:1000
WB: 1 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Immunohistochemical staining of human prostate shows strong cytoplasmic immunoreactivity in glandular cells.
Immunofluorescence staining in HeLa cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Target


Alternative Names
HSP90B1; heat shock protein 90kDa beta (Grp94), member 1; ECGP; GP96; TRA1; GRP94
Entrez Gene ID
UniProt ID

Citations


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References


Liu, B; Li, ZH; et al. Endoplasmic reticulum HSP90b1 (gp96, grp94) optimizes B-cell function via chaperoning integrin and TLR but not immunoglobulin. BLOOD 112:1223-1230(2008).
Rosenbaum, M; Andreani, V; et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. GENES & DEVELOPMENT 28:1165-1178(2014).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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