Rabbit Anti-Human HSF1 (a.a. 184-529) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

Recombinant human HSF1 protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 184-529 aa of BC014638

Conjugate

Application Notes

WB: 1:500-1:1000
IHC: 1:200-1:800

General Notes

This product (Catalog No. DPABH-21166) is a rabbit-derived polyclonal antibody raised against human HSF1, targeting the amino acid region 184–529 of the protein. This antibody exhibits cross-reactivity with Human HSF1 from mouse. It has been affinity purified by Protein A. HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).
DPABH-21166 is suitable for use in Western blot (WB) (1:500–1:1,000), IHC (1:200–1:800) applications.
DPABH-21166 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

Heat shock factor protein 1; HSF 1; HSF1; HSTF 1; HSTF1

Entrez Gene ID

UniProt ID