General Notes
This product (Catalog No. DPABH-23617) is a rabbit-derived polyclonal antibody raised against human HRH1, targeting the amino acid region 212–416 of the protein. It has been affinity purified by Protein A. The histamine H1 receptor (H1R) is a G protein-coupled receptor (GPCR) encoded by the HRH1 gene located on chromosome 3p25.3 in humans, serving as the primary mediator of histamine's effects on smooth muscle contraction, increased vascular permeability, catecholamine release, and neurotransmission in both central and peripheral tissues. Structurally, H1R features seven transmembrane domains typical of GPCRs and couples to Gq proteins upon histamine binding, activating phospholipase C to hydrolyze phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate and diacylglycerol, which in turn elevate intracellular calcium levels and stimulate protein kinase C activity. This signaling cascade underpins key physiological responses, including vasodilation, bronchoconstriction, pruritus, and enhanced endothelial permeability, contributing to immediate hypersensitivity reactions. The receptor is ubiquitously expressed in neurons, airway smooth muscle cells, vascular endothelial cells, and immune cells such as mast cells, with particularly high density in the brain, lungs, and skin.
DPABH-23617 is suitable for use in ELISA, IHC, Western blot (WB) applications.
DPABH-23617 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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