Mouse Anti-Human HIV-1 gp41 Hybridoma [NI-TWN36] (CSC-H0639)

This hybridoma produces mAbs (IgG1, kappa light chain) against human HIV-1 gp41 Datasheet

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General Information

Lysates of purified HIV-1
IgG1, kappa light chain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with lysates of purified HIV-1. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts specifically with HIV-1 gp41.
Growth Properties

Culture Method

Complete Growth Medium
DMEM with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, supplemented with 20% FBS
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO


The Human T-lymphotropic virus Type I (HTLV-1) is a human RNA retrovirus that is known to cause a type of cancer, referred to as adult T-cell leukemia and lymphoma, and a demyelinating disease called HTLV-I associated myelopathy/Tropical spastic paraparesis (HAM/TSP). HTLV-I is one of a group of closely related primate T lymphotropic viruses (PTLVs). Members of this family that infect humans are called Human T-lymphotropic viruses, and the ones that infect old-world primates are called Simian T-lymphotropic viruses. To date, four types of HTLVs (HTLV-I, HTLV-II, HTLV-III, and HTLV-IV) and four types of STLVs (STLV-I, STLV-II, STLV-III, and STLV-V) have been identified. The HTLVs are believed to originate from intraspecies transmission of STLVs. The original name for HIV, the virus that causes AIDS, was HTLV-III; this term is no longer in use . The HTLV-1 genome is diploid, composed of two copies of a single-stranded RNA virus whose genome is copied into a double-stranded DNA form that integrates into the host cell genome, at which point the virus is referred to as a provirus. A closely related virus is bovine leukemia virus BLV.


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