GeneralInformation
Immunogen
Lysates of purified HIV-1
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with lysates of purified HIV-1. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts specifically with HIV-1 gp41.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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Schanz, M; Liechti, T; et al. High-Throughput Sequencing of Human Immunoglobulin Variable Regions with Subtype Identification. PLOS ONE 9:-(2014).
Ye, L; Wen, ZY; et al. Induction of HIV Neutralizing Antibodies against the MPER of the HIV Envelope Protein by HA/gp41 Chimeric Protein-Based DNA and VLP Vaccines. PLOS ONE 6:-(2011).
COA
Certificate of Analysis
