Specifications
Species Reactivity
Mouse; Chicken; Hamster; Cow; Human; Fruit fly; Oikopleura; Marmoset
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.
Applications
Application Notes
IHC/IF: 2.5 μg/ml; WB: 0.5 - 1 μg/ml.
Target
Alternative Names
HIST1H3A; histone cluster 1, H3a; H3 histone family, member A , H3FA, histone 1, H3a; histone H3.1; H3/A; histone H3/a; histone H3/b; histone H3/c; histone H3/d; histone H3/f; histone H3/h; histone H3/i; histone H3/j; histone H3/k; histone H3/l; histone
Product Background
Antigen Description
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Pathway
Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Gene Expression, organism-specific biosystem; Hemostasis, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Recombination, organism-specific biosystem;
Citations
Publication ()
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H3K9ac involved in the decondensation of spermatozoal nuclei during spermatogenesis in Chinese mitten crab Eriocheir sinensis
Genliang Li, Xianjiang Kang, Shumei Mu, Mingshen Guo, Shiwen Huang, Qinna Chen, Song Nong, Xiaomin Huang, Hongliu Hu & Ke Sun
Applications: ICC,IF
Reactive species: E. sinensis
"Abstract: As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding of the nuclear decondensation of the spermatozoa in E. sinensis. Using specific antibodies, we followed the structural distribution and acetylated lysine 9 of the histone H3 during spermatogenesis, especially spermiogenesis, of E. sinensis. Various spermary samples at different developmental stages were used for histological immunofluorescence and ultrastructural immunocytochemistry. Our results demonstrate a wide distribution of the histone H3 and H3K9ac during spermatogenesis, including spermatogonia, spermatocytes, spermatids, and immature and mature spermatozoa except for absence of H3K9ac in the secondary spermatocytes. Especially during the initial stage of nuclear decondensation, histone H3 lysine 9 was acetylated and then an amount of H3K9ac was removed from within to outside of the nuclei of late spermatids. The portion of remaining H3K9ac was gradually transferred from the nuclei during the stages of spermatozoa maturation. Our findings suggest both the acetylation of histone H3 lysine 9 and the remain of H3K9ac to contribute to the nuclear decondensation in spermatozoa of E. sinensis."
Article snippet: Rat anti-histone H3 monoclonal antibody (DCABH-50) and anti-H3K9ac produced in rabbit, affinity isolated antibody (H9286) were purchased from Creative Diagnostics (Shirley, NY, USA) and *, respectively. 
Figure 1. Localization of histone H3 and H3K9ac (acetyl-H3 lysine 9) in the spermatogonia in E. sinensis.
COA
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