Anti-HGS monoclonal antibody, clone 4I8

Antibody Isotype

IgG2b

Clone

4I8

Species Reactivity

Human

Immunogen

Recombinant full length Human HGS (amino acids 1-777) produced in HEK293T cells (NP_004703).

Conjugate

Application Notes

WB: 1/4000; ICC/IF: 1/100;

Alternative Names

HGS; hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Vps27; ZFYVE8; protein pp110; human growth factor-regulated tyrosine kinase substrate; HRS; FLJ11421; FLJ39878;

Entrez Gene ID

UniProt ID

Antigen Description

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Pathway

CXCR4-mediated signaling events, organism-specific biosystem; Disease, organism-specific biosystem; EGFR downregulation, organism-specific biosystem; ESCRT-0 complex, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystem;