Anti-Gliadin sIgA ELISA Kit (DEIA9968)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Species Reactivity
Intended Use
The assay is intended for the quantitative determination of anti-gliadin-sIgA antibodies in stool. For in vitro diagnostic use only.
Contents of Kit
1. One holder with precoated strips, 12 x 8 wells
2. ELISA wash buffer concentrate 10x, 2 x 100 mL
3. Conjugate (Peroxidase-labeled), 1 x 15 mL
4. Control negative, lyophilized, 4 vials
5. Control positive, lyophilized, 4 vials
6. Cut-off Control, lyophilized, 4 vials
7. TMB substrate (Tetramethylbenzidine), 1 x 15 mL
8. ELISA stop solution, 1 x 15 mL
9. Extraction buffer concentrate, 2.5x, 1 x 100 mL
The reagents are stable up to the expiry date stated on the label when stored at 2-9°C. For more detailed information, please download the following document on our website.
36.2 U/L
General Description
The celiac/coeliac disease is caused by the gliadin fraction of wheat gluten and similar proteins of rye and barley. The disease is manly manifested as chronic digestion insufficiency in children or young adults. In addition, patients with celiac disease have a greatly increased risk of developing malignant T-cell lymphoma of the small bowel, as T-cell lymphoma was found in 6 of 10 patients with coeliac sprue. Early diagnosis of celiac disease is important, because there is evidence that a gluten-free diet might help to prevent complications and malignancies (including intestinal lymphoma).
The measurement of anti-gliadin-sIgA-antibodies may be useful as a screening criterion before jejunal biopsy, the "Gold Standard", and for the monitoring of the gluten-free diet treatment.
1. Celiac disease
2. Food intolerance


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Constitutive alterations in vesicular trafficking increase the sensitivity of cells from celiac disease patients to gliadin (vol 2, 190, 2020)


Authors: Lania, Giuliana; Nanayakkara, Merlin; Maglio, Mariantonia; Auricchio, Renata; Porpora, Monia; Conte, Mariangela; De Matteis, Maria Antonietta; Rizzo, Riccardo; Luini, Alberto; Discepolo, Valentina; Troncone, Riccardo; Auricchio, Salvatore; Barone, Maria Vittoria

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples


Authors: Marin-Barroso, Evelyn; Messina, German A.; Bertolino, Franco A.; Raba, Julio; Pereira, Sirley V.

The gluten-free diet is a unique, effective treatment for different conditions related to gluten consumption. Therefore, it is crucial the availability of new methodologies for the sensitive and specific determination of gluten content in food samples. Herein, a screen printed electrode modified with carbon nanofibers coupled to a paper immunoaffinity platform was reported for the determination of gliadin in foods samples. The paper microzone covalently functionalized with specific anti-gliadin antibodies was placed on the modified electrode surface for the electrochemical determination of gliadin. The surface of the electrode modified with carbon nanofibers was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), which showed the improved sensitivity of the modified surface. The developed device was evaluated using different flour samples obtaining a favorable response. The calculated limit of detection for the device in analyzed samples was 0.005 mg kg(-1) and for the enzymelinked immunosorbent assay was 1.5 mg kg(-1). The coefficient of variation (CV) for the determination of 20 mg kg(-1) of gliadin was 4.11%. The disposable electrochemical sensor developed, represents an easyto- use and low-cost strategy for the determination of gliadin in food samples.

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