Anti-Gliadin ELISA Kit (DEIA1971)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
Anti-Gliadin is an indirect solid phase enzyme immunoassay (ELISA) for measurement of IgG and IgA class autoantibodies against Gliadin in human serum or plasma.
Contents of Kit
1. Divisible microplate
2. Combined Calibrators
3. Anti-Gliadin Controls
4. Sample buffer
5. Enzyme conjugate solution
6. TMB substrate solution
7. Stop solution
8. Wash solution
Storage
Store the kit at 2-8°C. Keep microwells sealed in a dry bag with desiccants. For more detailed information, please download the following document on our website.

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References


Exercise-induced allergic reactions on desensitization to wheat after rush oral immunotherapy

ALLERGY

Authors: Furuta, Tomoko; Tanaka, Kajiyo; Tagami, Kazunori; Matsui, Teruaki; Sugiura, Shiro; Kando, Naoyuki; Kanie, Yuuki; Naito, Michihiro; Izumi, Hidehiko; Tanaka, Akira; Sjolander, Sigrid; Yokooji, Tomoharu; Matsuo, Hiroaki; Ito, Komei

Background The effect of oral immunotherapy (OIT) on wheat allergy is promising in terms of the potential to obtain desensitization; however, the frequency of exercise-induced allergic reactions on desensitization (EIARDs) and the associated risk factors remain to be determined. Methods Twenty-five patients underwent rush OIT for wheat allergy, and 21 achieved the full-dose intake of wheat products (5 g of wheat protein). Exercise-provocation tests were repeatedly performed after the ingestion of a full-dose wheat product. The time-course of the levels of the specific IgEs (sIgE) to wheat extract, total gliadin, deamidated gliadin, recombinant gliadin components (alpha/beta-, gamma- and omega-5-), and glutenin (high and low molecular weight) components was analyzed using ImmunoCAP (R), ELISA, or IgE immunoblotting. Results Fourteen patients (66.7%) were diagnosed as EIARD+, which remained 5 years after rush OIT in 11 patients (52.4%). There were no differences in the clinical backgrounds of the EIARD+ and EIARD- patients. However, EIARD+ patients showed significantly higher sIgE levels to all gliadin and glutenin components than EIARD- patients before OIT. The sIgE levels to each component decreased equally after 1 and 2 years of OIT. On IgE immunoblotting, sera from all patients reacted to the multiple gluten bands, and some reacted to the water-soluble bands. The intensity of all IgE-reactive bands also became equally lighter after OIT. Conclusions EIARDs were frequently observed and remained for a long period after successful OIT for wheat allergy. None of the specific wheat components were found to contribute to EIARDs.

Celiac disease and reproductive failures: An update on pathogenic mechanisms

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

Authors: Di Simone, Nicoletta; Gratta, Matteo; Castellani, Roberta; D'Ippolito, Silvia; Specchia, Monia; Scambia, Giovanni; Tersigni, Chiara

Celiac disease (CD) is an autoimmune disorder that occurs in genetically predisposed people in which the ingestion of gluten leads to damage in the small intestine that clinically presents with malabsorption-related symptoms. CD can also be the underlying cause of several non-gastrointestinal symptoms. This review summarizes evidence on the relationship between CD and gynecological/obstetric disorders like reproductive failures. Although much has been reported on such a linkage, the pathogenic mechanisms remain unclear, especially those underlying extra-gastrointestinal clinical manifestations. Studies conducted on celiac subjects presenting gynecological/obstetric disorders have pointed to intestinal malabsorption, coagulation alterations, immune-mediated tissue damage, and endometrial inflammation as the main responsible pathogenic mechanisms. Currently, however, the knowledge of such mechanisms is insufficient, and further studies are needed to gain a more thorough understanding of the matter.

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