Magic™ Anti-GST tag antibody, clone wqh77

Objective: We investigated the null polymorphism in GSTM1 and GSTT1 genes and the antioxidant levels in oral submucous fibrosis (OSMF), leukoplakia and oral cancer patients along with healthy controls in a South Indian cohort. Methods: Genotyping was done using multiplex PCR and the antioxidant levels were estimated using biochemical methods Association between genotypes and different diseased states was determined by odds ratio with 95% confidence interval (CI) and chi-square analysis and for antioxidant levels student's t-test was used. Results: The relative risk for GSTM1 and GSTT1 gene polymorphisms was statistically insignificant for the 3 patient groups vs. controls. Comparing the frequency of the null genotypes between the groups of patients only GSTM1 polymorphism revealed a significant difference between OSMF & oral cancer subjects (p = 0.02). Further, analysis of the antioxidant parameters shows ceruloplasmin levels to be significantly elevated between patient groups and controls and among OSMF vs. cancer patients (p < 0.05). Similarly, malondialdehyde and glutathione levels were found to be significantly elevated among cancer and both cancer and leukoplakia subjects respectively in comparison with controls (p < 0.05). Analysis between the patient groups revealed glutathione levels to be significantly elevated between OSMF vs. cancer patients and cancer vs. leukoplakia patients (p < 0.05). Conclusion: In conclusion, this study finds GSTM1 null genotype and antioxidants (ceruloplasmin and glutathione levels) to be significantly higher in oral cancers than in precancerous lesions, and suggesting that they might be associated with the malignant transformation of the oral precancers. (C) 2017 Asian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Published by Elsevier Ltd. All rights reserved.

Background: Previous studies about the correlation of genetic polymorphisms in the multigene family of cytochrome P450 (CYPs), the effect of tobacco smoking, and the risk of developing cancer have been well investigated in different populations, but not in Iraq. Furthermore, the studies of malignance occurrence relationship with cigarette tobacco smoking revealed the presence of strong association, however, little is known about the risk of Waterpipe (WP) tobacco smoking. Thus, determination two important genetic polymorphisms in CYP1A1, a main member of CYPs, among Iraqi men was our first aim. This is the first study that highlights the correlation of CYP1A1 polymorphisms with the risk of lung cancer in Iraq. The second aim was to evaluate the combined association of WP tobacco smoking and CYP1A1-Ile(462)Val and-MspI polymorphisms in lung cancer risk. Methods: This study included 123 lung cancer patients and 129 controls. To determine the variant genotypes, the techniques of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and DNA sequencing were carried out. Results: The data revealed the possible associations of variant (G) allele of CYP1A1-Ile(462)Val (OR= 1.6; 95% CI= 1.1-2.4; P= 0.01) and variant (C) allele of CYP1A1-MspI (OR= 1.9; 95% CI= 1.3-2.7; P < 0.01) with the risk of lung cancer. The variant genotypes of CYP1A1 polymorphisms were significantly correlated in the case of squamous cell carcinoma and synergistically associated in the case of combined effect with WP tobacco smoking (ORIleVal462 = 2.0; 95% CI= 1.0-4.1; P= 0.04, and ORMspI = 2.6; 95% CI= 1.3-5.5; P <= 0.01). Conclusion: The results suggest that WP tobacco smoking and genetic polymorphisms in CYP1A1 are most likely important risk factors for lung cancer in the Iraqi population.