Anti-GCLM monoclonal antibody (DCABH-4685)

Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
BU3E23
Species Reactivity
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-274 of Human GCLM, purified from E. coli (NP_002052).
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/3000;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
GCLM; glutamate-cysteine ligase, modifier subunit; GLCLR; glutamate--cysteine ligase regulatory subunit; gamma glutamylcysteine synthetase; GCS light chain
Entrez Gene ID
UniProt ID

Product Background


Gene summary
GCLM (Glutamate-Cysteine Ligase Modifier Subunit) is a Protein Coding gene. Diseases associated with GCLM include myocardial infarction and gamma-glutamylcysteine synthetase deficiency. Among its related pathways are Metabolism and Phase II conjugation. GO annotations related to this gene include protein heterodimerization activity and glutamate-cysteine ligase activity. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antigen Description
This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi apparatus. The encoded protein is organized with conserved oligomeric Golgi complex components 5, 7 and 8 into a sub-complex referred to as lobe B. Alternative splicing results in multiple transcript variants. Protein Symbol:P48507-GSH0_HUMANRecommended name:Glutamate--cysteine ligase regulatory subunitProtein Accession:P48507Secondary Accessions:A8K334D3DT45M5A959Q6FHC1Q9NPX9Q9NU74Glutamate-cysteine ligase regulatory subunit is an enzyme that in humans is encoded by the GCLM gene. 0The function about GCLM antigen include contributes_to glutamate-cysteine ligase activity; contributes_to glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding; protein heterodimerization activity.
Pathway
Biological oxidations, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, organism-specific biosystem; glutathione, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem.

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Cheng, LL; Li, B; et al. Identification of an E-box motif as a transcriptional repressor element in the proximal promoter region of the GCLC gene in rat lung epithelial L2 cells. FREE RADICAL BIOLOGY AND MEDICINE 39:1030-1040(2005).
Morzadec, C; Bouezzedine, F; et al. Inorganic arsenic impairs proliferation and cytokine expression in human primary T lymphocytes. TOXICOLOGY 300:46-56(2012).

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