Rabbit Anti-GCAT Polyclonal antibody

The purpose of this study were to prepare the novel supported bimetallic cobalt-nickel catalysts on the core-shell magnetic nanocomposite of activated carbon derived from wood by sequential and co-deposition-precipitation. The performance of the prepared catalyst was evaluated for the hydrogen generation from hydrolysis of sodium borohydride. The magnetic catalysts were characterized by applying the XRD, XPS, FTIR, FESEM, TEM, ICP, BET and VSM tests. The hydrogen generation rate was increased with the reduction of calcination temperature. The well dispersed magnetic nanoparticles were fabricated with average size below of 30 nm which was confirmed by TEM, FESEM and XRD results. The activity of the prepared samples with respect to the preparation method was illustrated to follow a specific order: Co/Ni/MWAC > Ni/Co/MWAC > Co-Ni/MWAC. The developed model derived from design of experiments could correlate the operating parameters with the experimental data while the correlation coefficient was achieved to be 0.99. The hydrogen generation rate was increased with increasing the reaction temperature and the concentration of sodium borohydride in the alkaline solutions. The hydrogen generation rate was measured to be 740.70 ml min(-1). gcat(-1) in the presence of the Co/Ni/MWAC at 30 degrees C. The experimental study also indicated that the hydrolysis of sodium borohydride was a zero order type reaction and the activation energy was calculated 40.70 kJ mol(-1). The stability of the prepared sample was also investigated for six cycles which showed the acceptable performance of the synthesized catalyst for the practical applications. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

In this study, Aeromonas salmonicida subsp. salmonicida was isolated, identified by 16S RNA sequencing and its potential lytic phage (ASP-1) was isolated and characterized. The bacterium was positive for virulence genes (ascV, fla, ahyB, gcaT, lip, alt and act) and phenotypic parameters (haemolysis, slime production, lipase activity, DNase test, gelatinase activity and protease activity) were tested. The bacterium was resistant to 27%, intermediate resistant to 14% and susceptible to 59% of tested common antibiotics. Transmission electron microscopy analysis revealed that lytic ASP-1 belongs to the Myoviridae family. The isolated phage was more specific against A. salmonicida subsp. salmonicida (efficiency of plating index=1), but also had infectivity to A. hydrophila lab strain 1. The bacteriolytic effect of ASP-1 was tested at early exponential phase culture of A. salmonicida subsp. salmonicida, and bacteria growth was apparently decreased with time and MOI dependent manner. One-step growth of ASP-1 showed approximately 30min of latent period, 16 PFU/infected cells of burst size and 40min of rise period. The adsorption rate was determined as 3.61x10(8)PFUmL(-1)min(-1) for 3min, and rate decreased with time. The ASP-1 genome size was estimated to be approximately 55-60kD. The phage was stable over wide-range of temperatures, pH and salinity, thus could withstand at severe environmental conditions, indicating that ASP-1 has a potential to develop as an alternative antibiotic touse in ornamental and aquaculture industry.