Anti-GAPDH monoclonal antibody (DMAB35346)
Rabbit Anti-GAPDH monoclonal antibody for WB, ICC/IF, IHC, IP, FC
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Human, Mouse, Rat, Monkey, Chicken, zebrafish
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC: 1:10-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/1,000 dilution.
Lane 1: NCCIT;
Lane 2: PC12
Lane 3: F9
Fig2: ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as secondary antibody
38 kDa BFA-dependent ADP-ribosylation substrate; aging associated gene 9 protein; Aging-associated gene 9 protein; BARS-38; cb609; EC 184.108.40.206
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Hasegawa, G; Kikuchi, M; et al. Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: An example of application for protein chemistry. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 63:33-42(2005).
Kuroki, A; Iyoda, M; et al. Th2 cytokines increase and stimulate B cells to produce IgG4 in idiopathic membranous nephropathy. KIDNEY INTERNATIONAL 68:302-310(2005).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.