Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth
ODONTOLOGY
Authors: Nakayama, Yohei; Inoue, Eiko; Kato, Ayako; Iwai, Yasunobu; Takai-Yamazaki, Mizuho; Tsuruya, Yuto; Yamaguchi, Arisa; Noda, Keisuke; Nomoto, Takato; Ganss, Bernhard; Ogata, Yorimasa
Abstract
Follicular dendritic cell-secreted protein (FDC-SP) is secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium (JE). Its expression could be controlled during inflammatory process of gingiva; however, responsible mechanism for gingival overgrowth and involvement of FDC-SP in clinical condition is still unclear. We hypothesized that JE-specific genes are associated with the initiation of drug-induced gingival enlargement (DIGE) called gingival overgrowth, and investigated the changes of JE-specific gene's expression and their localization in overgrown gingiva from the patients. Immunohistochemical analysis revealed that the FDC-SP localization was spread in overgrown gingival tissues.FDC-SPmRNA levels in GE1 and Ca9-22 cells were increased by time-dependent nifedipine treatments, similar to other JE-specific genes, such asAmelotin(Amtn) andLaminin beta 3subunit (Lam beta 3), whereastype 4 collagen(Col4) mRNA levels were decreased. Immunocytochemical analysis showed that FDC-SP, AMTN, and Lam beta 3 protein levels were increased in GE1 and Ca9-22 cells. Transient transfection analyses were performed using luciferase constructs including various lengths of humanFDC-SPgene promoter, nifedipine increased luciferase activities of -345 and -948FDC-SPconstructs. These results raise the possibility that the nifedipine-induced FDC-SP may be related to the mechanism responsible for gingival overgrowth does not occur at edentulous jaw ridges.
Synthesis, O-2-binding ability and catalytic oxidation performance of cobalt(II) complexes with dihydroxamic acid functionalized N-pivot lariat ethers
CHINESE CHEMICAL LETTERS
Authors: Wei, Xing-Yue; Wang, Xing-Min; Li, Ning; Qin, Sheng-Ying
Abstract
Novel Co(II) complexes with dihydroxamic acids functionalized N-pivot lariat ether CoL1-CoL4 were synthesized and characterized. Their oxygenation constants (In K-o2) and thermodynamic parameters (Delta H degrees, Delta S degrees) were measured. And their catalytic performance in oxidation of p-xylene to p-toluic acid (PTA) was investigated. The enhancement of O-2-binding and catalytic oxidation activity by the oxa aza crown ether ring of the cobalt complexes were discussed. (C) 2014 Xing-Yue Wei. Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. All rights reserved.
COA
Publication 
Loading ......
