Anti-FEN1 (aa 1-100) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FEN1.

Conjugate

Application Notes

ICC/IF: 1 μg/ml; WB: 1 μg/ml; IHC-P: 1 μg/ml.

Alternative Names

FEN1; flap structure-specific endonuclease 1; RAD2; flap endonuclease 1; DNase IV; FEN 1; maturation factor 1; MF1; maturation factor-1; FEN-1;

Entrez Gene ID

UniProt ID

Antigen Description

Structure-specific nuclease with 5-flap endonuclease and 5-3 exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5-end of a downstream Okazaki fragment. It enters the flap from the 5-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5-3 exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Pathway

Base Excision Repair; Base excision repair; Cell Cycle; Cell Cycle, Mitotic; Chromosome Maintenance; DNA Repair;