Rabbit Anti-Human FCHO2 Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

A synthesized peptide derived from human FCHO2.

Conjugate

Application Notes

WB 1:500-1:2000
IHC 1:50-1:200
IF/ICC 1:100-1:500

General Notes

This product (Catalog No. DPABH-20710) is a rabbit-derived polyclonal antibody raised against human FCHO2. This antibody exhibits cross-reactivity with FCHO2 from mouse. It has been affinity purified by Protein A. Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.
DPABH-20710 is suitable for use in WB 1 (500-1:2000), IHC 1 (50-1:200), IF/ICC 1 (100-1:500) applications.
DPABH-20710 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID