Anti-Euplotes aediculatus α-, β- & γ- Plateins monoclonal antibody (DMAB9239)

Mouse anti-Euplotes aediculatus α-, β- & γ- Plateins monoclonal antibody for IHC, WB, IHC Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
PL-3
Species Reactivity
N/A
Immunogen
β- & γ- plateins (not α-)
Conjugate
Unconjugated

Target


Alternative Names
Plateins; β platein; γ platein

Product Background


Antigen Description
Protistan cells employ a wide variety of strategies to reinforce and give pattern to their outermost cortical layers. The hypotrich ciliate Euplotes possesses a continuous monolayer of scales or plates, located within flattened membranous sacs (`alveoli") just below the plasma membrane, and this provides rigidity and form to the cell. Using immunological techniques, the major proteins comprising these `alveolar plates" have been identified and termed α-, β-, and γ -plateins. The present report describes work leading to the molecular characterization of three plateins, α1 and α2 (predicted Mrs of 61 and 56 kDa) and a β/γ form (Mr=73 kDa). All three proteins have features that are hallmarks of articulins, a class of cytoskeletal proteins that has been identified in the cortex of a wide variety of protistan cells, including certain flagellates, ciliates, dinoflagellates and Plasmodium. Chief among these common features are a prominent primary domain of tandem 12-amino acid repeats, rich in valine and proline, and a secondary domain of fewer, shorter repeating units. However, variations in amino acid use within both primary and secondary repetitive domains, and a much more acidic character (predicted pIs of 4. 42555. 9), indicate that the plateins represent the first proteins in a new subclass or family of articulins.

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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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