Anti-eIF3J monoclonal antibody (DCABH-11384) Made to order

Rabbit anti-Human eIF3J monoclonal antibody for WB, ELISA

View other eIF3J antibodies

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
A synthetic peptide of human EIF3J is used for rabbit immunization.
Conjugate
Unconjugated

Target


Alternative Names
EIF3J; eukaryotic translation initiation factor 3, subunit J; EIF3S1, eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 3 subunit J; eIF3 alpha; eIF3 p35
Entrez Gene ID
UniProt ID

Product Background


Gene summary
EIF3J (Eukaryotic Translation Initiation Factor 3 Subunit J) is a Protein Coding gene. Among its related pathways are Gene Expression and Transport to the Golgi and subsequent modification. GO annotations related to this gene include translation initiation factor activity. This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antigen Description
Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al. , 2004 [PubMed 14688252]; Fraser et al. , 2007 [PubMed 17588516]). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA. Eukaryotic translation initiation factor 3 subunit J is a protein that in humans is encoded by the EIF3J gene. 0The function about eIF3J antigen include protein binding; contributes_to translation initiation factor activity.
Pathway
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of a pool of free 4S subunits, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 6S ribosomal subunit, organism-s.

Citations


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References


Perard, J; Rasia, R; et al. Human initiation factor eIF3 subunit b interacts with HCV IRES RNA through its N-terminal RNA recognition motif. FEBS LETTERS 583:70-74(2009).
Fraser, CS; Lee, JY; et al. The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro. JOURNAL OF BIOLOGICAL CHEMISTRY 279:8946-8956(2004).

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