Anti-ECT2 monoclonal antibody (DCABH-11356) Made to order

Rabbit anti-Human ECT2 monoclonal antibody for WB, ELISA

View other ECT2 antibodies

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
A synthetic peptide of human ECT2 is used for rabbit immunization.
Conjugate
Unconjugated

Target


Alternative Names
ECT2; epithelial cell transforming sequence 2 oncogene; protein ECT2; ARHGEF31; epithelial cell-transforming sequence 2 oncogene; FLJ10461
Entrez Gene ID
UniProt ID

Product Background


Gene summary
ECT2 (Epithelial Cell Transforming 2) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Signaling by Rho GTPases. GO annotations related to this gene include protein homodimerization activity and GTPase activator activity. An important paralog of this gene is TOPBP1. The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding two different isoforms have been found for this gene.
Antigen Description
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. Protein ECT2 is a protein that in humans is encoded by the ECT2 gene. The function about ECT2 antigen include GTPase activator activity; Rho GTPase binding; Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; protein binding; protein homodimerization activity; signal transducer activity.
Pathway
Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; G alpha (12/13) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; NRAGE signals death through JNK, organism-specific biosystem; PLK1 signaling events, organism-specific biosystem; Regulation of RhoA activity, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem.

Citations


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References


Matthews, HK; Delabre, U; et al. Changes in Ect2 Localization Couple Actomyosin-Dependent Cell Shape Changes to Mitotic Progression. DEVELOPMENTAL CELL 23:371-383(2012).
Morita, K; Hirono, K; et al. The Caenorhabditis elegans ect-2 RhoGEF gene regulates cytokinesis and migration of epidermal P cells. EMBO REPORTS 6:1163-1168(2005).

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