Dengue virus type 3 antigens prepared from infected mouse brain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8
Clone
6E5-22
Cell Line Description
Animals were immunized with dengue virus type 3 antigens prepared from infected mouse brains (the H87 strain was used). The antibody is type specific. Spleen cells were fused with P3X63Ag8 myeloma cells. Antibodies should be prepared as ascites in pristane primed BALB/c mice. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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COA
Certificate of Analysis
