Rabbit Anti-DVL1 Polyclonal Antibody (DPABH-24526)
Rabbit Anti-Human DVL1 Polyclonal Antibody for WB, IHC
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Human, Mouse, Rat
Recombinant funsion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of various cell lines, using DVL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
DVL1; dishevelled segment polarity protein 1; DVL; DVL1L1; DVL1P1; segment polarity protein dishevelled homolog DVL-1
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Katoh, M; et al. WNT/PCP signaling pathway and human cancer (review). ONCOLOGY REPORTS 14:1583-1588(2005).
You, XJ; Bryant, PJ; et al. Expression of Wnt pathway components frizzled and disheveled in colon cancer arising in patients with inflammatory bowel disease. ONCOLOGY REPORTS 18:691-694(2007).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.