Rabbit Anti-Human DRD1 (a.a. 338-446) Polyclonal antibody

Name: Rabbit Anti-Human DRD1 (a.a. 338-446) Polyclonal antibody
SKU: DPABH-23454
Rating: 5 (1 reviews)

Rabbit Anti-Human DRD1 (a.a. 338-446) Polyclonal antibody for WB, IF-Fro, ELISA

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COA

SDS

Datasheet

Key features and details

Target: Human DRD1
Host: Rabbit
Application: WB, IF-Fro, ELISA
Species Reactivity: Human, Mouse, Rat

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

Recombinant human DRD1 protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 338-446 aa of BC074978

Conjugate

Unconjugated

Applications

General Notes

This product (Catalog No. DPABH-23454) is a rabbit-derived polyclonal antibody raised against human DRD1, targeting the amino acid region 338–446 of the protein. This antibody exhibits cross-reactivity with Human DRD1 from mouse and rat. It has been affinity purified by Protein A. Dopamine is a neurotransmitter that plays a crucial role in physical and mental health, such as cardiovascular, hormonal, renal, and central nervous systems (PMID: 29220802). Five subtypes of mammalian dopamine receptors are grouped into two classes, the D1- and D2-like classes. The D1-like class includes D1 and D5 receptors whereas the D2-like class includes D2, D3, D4 subtypes (PMID: 9457173). Dopamine receptor D1 (DRD1) is the most abundant form of dopamine receptor in the central nervous system. DRD1 stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses (PMID: 1977312). DRD1 has a calculated molecular weight of 49 kDa, larger apparent molecular weight of 60-80 kDa may be due to glycosylation (PMID: 1281547; 23821371).
DPABH-23454 is suitable for use in Western blot (WB), IF-Fro, ELISA applications.
DPABH-23454 is supplied as a liquid formulation in PBS only, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

D(1A) dopamine receptor; DADR; Dopamine D1 receptor; dopamine receptor D1

Entrez Gene ID

1812

UniProt ID

P21728

Citations

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Documents

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Customer Reviews

References

Analysis of the Promoter Region of Human Dopamine Receptor D1

JOURNAL OF MOLECULAR NEUROSCIENCE

Authors: Wu, Xue; Xu, Feng-ling; Wang, Bao-jie; Yao, Jun

Abstract

Dysregulation of dopamine receptor D1 (DRD1) is involved in multiple neuropsychiatric disorders. The 5' regulatory region of DRD1 has not been characterized fully. We applied the luciferase assay and the electrophoretic mobility shift assay to explore the activity of the 5' regulatory region of DRD1 in SH-SY5Y and 293T cells. We found that the promoter region of DRD1 corresponded to positions - 1250 to + 250 in the DNA sequence, and the putative core promoter region was from - 113 to + 250 (transcriptional start site of exon, +1). The sequence 5'-gggacgcgcgggcggggtgggctgtgccccgcgggaaccccgccggcctgtgcgcttgctg-3' was identified as a possible transcription factor-binding domain. Further research is warranted to explore the function of the 5' regulatory region of DRD1.

Social rank-associated stress vulnerability predisposes individuals to cocaine attraction

SCIENTIFIC REPORTS

Authors: Yanovich, Chen; Kirby, Michael L.; Michaelevski, Izhak; Yadid, Gal; Pinhasov, Albert

Abstract

Studies of personality have suggested that dissimilarities in ability to cope with stressful situations results in differing tendency to develop addictive behaviors. The present study used selectively bred stress-resilient, socially-dominant (Dom) and stress-vulnerable, socially-submissive (Sub) mice to investigate the interaction between environmental stress and inbred predisposition to develop addictive behavior to cocaine. In a Conditioned Place Preference (CPP) paradigm using cocaine, Sub mice displayed an aversion to drug, whereas Dom mice displayed drug attraction. Following a 4-week regimen of Chronic Mild Stress (CMS), Sub mice in CPP displayed a marked increase (>400%) in cocaine attraction, whereas Dom mice did not differ in attraction from their non-stressed state. Examination of hippocampal gene expression revealed in Sub mice, exposure to external stimuli, stress or cocaine, increased CRH expression (>100%), which was evoked in Dom mice only by cocaine exposure. Further, stress-induced decreases in DRD1 (>60%) and DRD2 (>50%) expression in Sub mice differed markedly from a complete lack of change in Dom mice. From our findings, we propose that social stratification dictates vulnerability to stress-induced attraction that may lead to addiction via differential regulation of hippocampal response to dopaminergic input, which in turn may influence differing tendency to develop addictive behaviors.