Mouse anti-DMPO Nitrone Adduct monoclonal antibody for ICC/IHC, IF
55 dimethyl 1 pyrroline N oxide nitrone adduct; DMPO Nitrone Adduct; 5,5-dimethyl-1-pyrroline N-oxide; DMPO; 5,5-DIMETHYL-1-PYRROLINE N-OXIDE; 2,2-DIMETHYL-3,4-DIHYDRO-2H-PYRROLE 1-OXIDE
The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
Towner, RA; Garteiser, P; et al. In vivo detection of free radicals in mouse septic encephalopathy using molecular MRI and immuno-spin trapping. FREE RADICAL BIOLOGY AND MEDICINE 65:828-837(2013).
Zuo, L; Chen, YR; et al. The Radical Trap 5,5-Dimethyl-1-Pyrroline N-Oxide Exerts Dose-Dependent Protection against Myocardial Ischemia-Reperfusion Injury through Preservation of Mitochondrial Electron Transport. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 329:515-523(2009).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.