Rabbit Anti-Human DCLRE1C (C-terminal) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

A synthesized peptide derived from human DCLRE1C, corresponding to a region within C-terminal amino acids.

Conjugate

Application Notes

WB 1:500-1:2000

General Notes

This product (Catalog No. DPABH-01382) is a rabbit-derived polyclonal antibody raised against human DCLRE1C. This antibody exhibits cross-reactivity with DCLRE1C from mouse and rat. It has been affinity purified by Protein A. Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
DPABH-01382 is suitable for use in WB 1 (500-1:2000) application.
DPABH-01382 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Entrez Gene ID

UniProt ID