Anti-Cysteine Sulfenic Acid polyclonal antibody

Background Thymol is a monoterpene phenol found in thyme species plants. The present study was carried out to investigate the effect of thymol and its molecular mechanism on non-small lung cancer (A549) cells. Methods The cytotoxic effect of thymol on A549 cells was assessed via MTT assay. ROS production, macromolecular damage, apoptosis were determined using DCF-DA, PI, AO/EtBr stains, respectively. ROS-dependent effect of thymol was confirmed using NAC. The expression of caspase-9, Bcl-2, Bax and cell cycle profile was analyzed via western blot and FACS, respectively. Results The antiproliferative effect of thymol on A549 cells was found to be both dose and time dependent with IC50 values of 112 mu g/ml (745 mu M) at 24 h. Thymol treatment favored apoptotic cell death and caused G0/G1 cell cycle arrest. It mediated cellular and nuclear morphological changes, phosphatidylserine translocation, and mitochondrial membrane depolarization. Additionally, upregulation of Bax, downregulation of Bcl-2, and apoptotic fragmented DNA were also observed. Thymol induced ROS by reducing the SOD level which was confirmed via in vitro and in silico analysis. Furthermore, the levels of lipid peroxides and protein carbonyl content were elevated in thymol-treated groups. Notably, N-acetyl cysteine pretreatment reversed the efficacy of thymol on A549 cells. Moreover, thymol-treated human PBMC cells did not show any significant cytotoxicity. Conclusion Overall, our results confirmed that thymol can act as a safe and potent therapeutic agent to treat NSCLC. [GRAPHICS] .

In the proposed method cysteine-functionalized chitosan-coated magnetic nanoparticles were synthesized and used as an effective adsorbent for the extraction of carboxin and bensulfuron-methyl. The experiments were performed according to an orthogonal rotatable Box-Behnken design. The results showed that the limits of detection, quantification values, and linear ranges were 0.6, 2.0 mu g.L-1, and 2-1000 mu gL(-1)for carboxin, and 0.3, 1.0 mu gL(-1), and 1-1000 mu gL(-1)for bensulfuron-methyl. The proposed method was applied to spiked real samples. The percentage of recovery values (91.53%-98.46%) with low relative standard deviation (<= 4.6%) demonstrated excellent repeatability and applicability of this adsorbent to water samples.