Anti-CRK monoclonal antibody, clone nBcdbn70834

Crk and CrkL adaptors play essential neuronal positioning roles downstream of Reelin-induced Dab1 tyrosine phosphorylation. Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1. Here using mass spectrometry, biochemical and mutational analyses we show that Dab1 suppresses Cin85 phosphorylation at Ser587. Furthermore a Cin85 Ser587 phosphomimetic disrupts the Dab1-Cin85 complex without affecting the Cin85-CapZ complex. These data provide an early glimpse into how Cin85 phosphorylation might alter the composition of its scaffolding partners to regulate its diverse roles including vesicular trafficking, receptor endocytosis and actin remodeling. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Background: Potassium (K) availability in soil and plant uptake is restrained by the dynamic interactions among the different pools of K. Aims: To understand these interactions, a study was undertaken to assess the quantity-intensity (Q/I) and buffering characteristics of rainfed maize (Zea mays L.) growing soils. Ten contrasting soils were evaluated for K partitioning changes in exchangeable K (Delta EK) and non-exchangeable K (Delta NEK) pools in the soil-solution phase and buffering characteristics using a modified version of Q/I approach. Results: The partitioned Q/I isotherms showed strong adsorption with the increase in K concentration ratio (CRK) and the changes due to Delta EK were higher than changes due to Delta NEK. Total buffering capacity (PBCK) significantly correlated (r = 0.92, p <0.01) with clay content with a major share contributed by buffering capacity owing to non-exchangeable K ( PBC Delta NEK K ) rather than exchangeable K ( PBC Delta EK K ). The fixation capacity (beta) factor, the magnitude of added K converted into a non-exchangeable pool, ranged from 41 to 63%, whereas release (alpha) factor, the magnitude of added K converted to the exchangeable pool, ranged from 19 to 36%. Both threshold solution K (CKr) and threshold exchangeable K (EKr) values were found to be high in Satran clay loam (S2) and lower in Doon silty clay loam (S3) soils. The equilibrium exchangeable K (EKo) was found close to minimum exchangeable K (E-min) in Doon silty clay loam (S3) and Babaweyl sandy clay loam (S1) soils and overall E-min constituted about 8.94 to 0.57% of the EKo. Conclusion: It may be concluded that K Q/I isotherm partitioning provides a valuable insight to assess the dynamic relations. The ratio of alpha/beta (K recharge index) could be used to evaluate the K enrichment capacity of soil to K additions while EKr and E-min can be potentially useful in the elucidation of exchangeable K as K fertility index especially in soils with poor K fertilizer management.