Anti-Cotinine monoclonal antibody, clone D58H60

Name: Anti-Cotinine monoclonal antibody, clone D58H60
SKU: DMAB7637
Rating: 5 (1 reviews)

Mouse anti-Human GFAP monoclonal antibody for IA

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COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG1

Clone

D58H60

Immunogen

Cotinine-BSA

Conjugate

Unconjugated

Target

Alternative Names

(5S)-1-methyl-5-(3-pyridyl)pyrrolidin-2-one; Cotinine; 1-METHYL-5-[3-PYRIDYL]-2-PYRROLIDINONE; L-COTININE; (-)-COTININE; COTININE; S-(-)-COTININE; (S)-(-)-1-METHYL-5-(3-PYRIDIYL)-2-PYRROLIDINONE; [S]-1-METHYL-5-[3-PYRIDYL]-2-PYRROLIDINONE; S(-)-1-METHYL-5

Citations

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All-in-one platform for salivary cotinine detection integrated with a microfluidic channel and an electrochemical biosensor

Kyungyeon Lee, Taehee Yoon, Hee-Seon Yang, Sunyeong Cha, Yong-Pil Cheon, Leila Kashefi-Kheyrabadi, Hyo-Il Jung

Lab Chip2020 JanPubMed ID: 31825049Read Article

Applications: Unspecified application
Reactive species: Human
"Abstract: Medical disorders caused by second-hand smoke are a major public health concern worldwide. To estimate the level of second-hand smoke exposure, salivary diagnostics for cotinine analysis is a compelling alternative in conventional diagnostics using bio-fluids, such as blood and urine, owing to its simple and non-invasive collection method. However, there are several critical issues, such as tedious multisteps, demand for expertise, and field unavailability to collect and transport the purified saliva for further analysis. Here, an all-in-one platform is presented to simply collect real human saliva and directly deliver it onto the biosensing surface. The platform consists of a commercial cotton-swab-type collector, 3D-printed housing, and microfluidic channel integrated with an electrochemical competitive immunosensor to evaluate the level of salivary cotinine. The immunosensor is based on a competitive binding assay between cotinine-conjugated horseradish peroxidase (C-HRP) and cotinine for anti-cotinine binding sites. The current responses obtained from the HRP-thionine-H2O2 system decreased proportionally to the cotinine concentration. This immunosensor successfully detected its target over a range of 1 × 10-1 to 1 × 104 pg ml-1 with a low limit of detection of 6 × 10-2 pg ml-1 and a limit of quantification of 1 × 10-1 pg ml-1. In addition, the platform is applicable to various commercial cotton-swab-type saliva collectors and can successfully transfer the saliva in wide flow rates ranging from 0.1 to 30 ml min-1 without leakage or damage to the sensing surface."
Article snippet: The mouse anti-cotinine and C-HRP were obtained from Creative Diagnostics (DMAB7637) and *, respectively.

Calibration curve for the detection of cotinine using immunosensor. (Kyungyeon Lee, <i>et al</i>, 2020)

Figure 1. Calibration curve of the immunosensor for cotinine detection

Documents

pdf COA

pdf SDS

pdf Datasheet

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References

DNA methylation level in blood and relations to breast cancer, risk factors and environmental exposure in Greenlandic Inuit women

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY

Authors: Wielsoe, Maria; Tarantini, Letizia; Bollati, Valentina; Long, Manhai; Bonefeld-Jorgensen, Eva Cecilie

Abstract

Several studies have found aberrant DNA methylation levels in breast cancer cases, but factors influencing DNA methylation patterns and the mechanisms are not well understood. This case-control study evaluated blood methylation level of two repetitive elements and selected breast cancer-related genes in relation to breast cancer risk, and the associations with serum level of persistent organic pollutants (POPs) and breast cancer risk factors in Greenlandic Inuit. DNA methylation was determined using bisulphite pyrosequencing in blood from 74 breast cancer cases and 80 controls. Using first tertile as reference, the following was observed. Positive associations for ATM in second tertile (OR: 2.33, 95% CI: 1.04; 5.23) and ESR2 in third tertile (OR: 2.22, 95% CI: 0.97; 5.05) suggest an increased breast cancer risk with high DNA methylation. LINE-1 methylation was lower in cases than controls. In third tertile (OR: 0.42, 95% CI: 0.18; 0.98), associations suggest in accordance with the literature an increased risk of breast cancer with LINE-1 hypomethylation. Among controls, significant associations between methylation levels and serum level of POPs and breast cancer risk factors (age, body mass index, cotinine level) were found. Thus, breast cancer risk factors and POPs may alter the risk through changes in methylation levels; further studies are needed to elucidate the mechanisms.

Death related to nicotine replacement therapy: A case report

FORENSIC SCIENCE INTERNATIONAL

Authors: Tjoncke, J-A; Goncalves, R.; Castaing, N.; Molimard, M.; Tovagliaro, F.; Titier, K.

Abstract

Transdermal nicotine patches and nicotine tablets are widely used for substitution therapies after cessation of smoking. Toxic concentrations of nicotine and cotinine, its main metabolite, are rarely reported, either in cases of misuse or in a fatal context. We report here a rare fatal case due to massive exposure to nicotine replacement therapy. A 41-year-old man was found dead by his cellmate with 7 nicotine patches on the body. There were 14 nicotine patches (21 mg) and 5 empty blisters of nicotine tablets (Nicopass (R) 1.5 mg) in the bin. External, internal, and histological examinations revealed asphyxia syndrome. Toxicological analyses indicated lethal concentrations of nicotine and cotinine in femoral (2239 and 1230 ng/mL) and cardiac blood (1344 and 1090 ng/mL). Screening for ethanol, drugs, and illicit drugs revealed therapeutic concentrations of cyamemazine, lormetazepam, nordiazepam, oxazepam, and buprenorphine and its metabolite. THC and its metabolites were also detected, re flecting use of cannabis. The findings highlight the risk of nicotine poisoning in persons using nicotine patches. This case emphasises the importance of carrying out complete toxicological analyses to prevent other instances of nicotine poisoning from being overlooked.(C) 2020 Elsevier B.V. All rights reserved.