Human Anti-Human Colon Tumor Associated Antigen (CTAA) Heterohybridoma [DP 99CW60-2] (CSC-H1212)

This hybridoma produces mAbs (IgG3, kappa light chain) against human Colon Tumor Associated antigen (CTAA)

General Information


Immunogen
Autologous tumor antigen
Isotype
IgG3, kappa light chain
Fusion Species
Human X Human/Mouse Heterohybridoma
Immunological Donor
Human
Clone
DP 99CW60-2
Cell Line Description
This transformed cell line was derived from B-cells of a patient undergoing surgical resection of colorectal cancer. The patient was actively immunized with autologous tumor antigen. Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line. CO 88BV59-1 cells produce monoclonal antibody 88BV59 that has been shown to recognize colon tumor associated antigen (CTAA) 16.88, also referred to as CTA #1. Both the 88BV59 antibody and the 16-88 antibody recognize the same tumor associated antigen, but react with different epitopes on that antigen.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
RPMI 1640
with 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose and 1500 mg/L sodium bicarbonate

, supplemented with 10% FBS.
Storage
Liquid nitrogen vapor phase.

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