Specifications
Species Reactivity
Human; Mouse; Rat; Dog; Pig
Immunogen
KLH conjugated synthetic peptide derived from human TLR3: 801-905/905
Applications
Application Notes
WB: 1:500-2000
IHC-P: 1:100-500
IHC-F: 1:100-500
IF: 1:100-500
General Notes
This product (Catalog No. CPBT-48145RC) is a rabbit-derived IgG polyclonal antibody raised against human TLR3, targeting the amino acid region 801–905 of the protein. This antibody exhibits cross-reactivity with TLR3 from mouse, rat, dog, and pig. It has been affinity purified by protein A. The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It thus plays a role in host defense against multiple viruses.
CPBT-48145RC is suitable for use in Western blot (WB) (1:500–1:2,000), IHC-P (1:100–1:500), IHC-F (1:100–1:500), IF (immunofluorescence) (1:100–1:500) applications.
CPBT-48145RC is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
Target
Alternative Names
Toll-like receptor 3; TLR3; CD283; CD283 antigen; TLR 3; Toll Like Receptor 3; TLR3_HUMAN
Citations
Publication ()
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Double-Stranded Ribonucleic Acid-Mediated Antiviral Response Against Low Pathogenic Avian Influenza Virus Infection
Ahmed-Hassan H, Abdul-Cader MS, Ahmed Sabry M, Hamza E, Sharif S, Nagy E, Abdul-Careem MF
Applications: IA
Reactive species: Chicken
"Abstract: Toll-like receptor (TLR)3 signaling pathway is known to induce type 1 interferons (IFNs) and proinflammatory mediators leading to antiviral response against many viral infections. Double-stranded ribonucleic acid (dsRNA) has been shown to act as a ligand for TLR3 and, as such, has been a focus as a potential antiviral agent in many host-viral infection models. Yet, its effectiveness and involved mechanisms as a mediator against low pathogenic avian influenza virus (LPAIV) have not been investigated adequately. In this study, we used avian fibroblasts to verify whether dsRNA induces antiviral response against H4N6 LPAIV and clarify whether type 1 IFNs and proinflammatory mediators such as interleukin (IL)-1b are contributing to the dsRNA-mediated antiviral response against H4N6 LPAIV. We found that dsRNA induces antiviral response in avian fibroblasts against H4N6 LPAIV infection. The treatment of avian fibroblasts with dsRNA increases the expressions of TLR3, IFN-α, IFN-β, and IL-1β. We also confirmed that this antiviral response elicited against H4N6 LPAIV infection correlates, but is not attributable to type 1 IFNs or IL-1β. Our findings imply that the TLR3 ligand, dsRNA, can elicit antiviral response in avian fibroblasts against LPAIV infection, highlighting potential value of dsRNA as an antiviral agent against LPAIV infections. However, further investigations are required to determine the potential role of other innate immune mediators or combination of the tested cytokines in the dsRNA-mediated antiviral response against H4N6 LPAIV infection."
Article snippet: For the immunostaining, we employed the same procedure described for staining for IFN-α and IFN-β, except the primary antibody, which was rabbit anti-chicken TLR3 polyclonal antibody (Creative Diagnostics, Shirley, NY) diluted at 1:200 in the blocking buffer. 
Figure 1. dsRNA increases TLR3 expression in avian fibroblasts.
COA
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