Human Anti-Human Cervical Carcinoma Hybridoma [DMO I22.5] (CSC-H0811)

This hybridoma produces mAbs (IgG1, kappa light chain) against human Cervical Carcinoma

General Information

IgG1, kappa light chain
Fusion Species
Human X Human Hybridoma
Immunological Donor
DMO I22.5
Cell Line Description
The fusion line is reported to be EBNA positive. Lymphocytes were isolated from a regional draining lymph node of a patient with squamous cell carcinoma of the cervix. B lymphocytes were fused with UC 729-6 cells . The antibody reacted with carcinomas of the cervix (CaSki, HeLa), prostate (PC-3), breast (ZR-76-1), colon (COLO-205) and melanoma (G-361) but was negative for normal fibroblasts (WI-38 and MRC-9), T. lymphocytes and peripheral blood lymphocytes.
Application Notes
In therapy and diagnosis, both in vitro and in vivo.
Growth Properties

Culture Method

Complete Growth Medium
RPMI 1640
with 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose and 1500 mg/L sodium bicarbonate

, supplemented with 10% FBS.
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Liquid nitrogen vapor phase.

Freezing medium: to complete growth medium, add 5%(v/v) DMSO


Cervical cancer is a malignant neoplasm arising from cells originating in the cervix uteri. One of the most common symptoms of cervical cancer is abnormal vaginal bleeding, but in some cases there may be no obvious symptoms until the cancer has progressed to an advanced stage.[1] Treatment usually consists of surgery (including local excision) in early stages, and chemotherapy and/or radiotherapy in more advanced stages of the disease.


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