Human Anti-Human Cervical Carcinoma Hybridoma [DMO I22.5] (CSC-H0811)

This hybridoma produces mAbs (IgG1, kappa light chain) against human Cervical Carcinoma Datasheet

Online Inquiry Add to basket

General Information


Isotype
IgG1, kappa light chain
Fusion Species
Human X Human Hybridoma
Immunological Donor
Human
Clone
DMO I22.5
Cell Line Description
The fusion line is reported to be EBNA positive. Lymphocytes were isolated from a regional draining lymph node of a patient with squamous cell carcinoma of the cervix. B lymphocytes were fused with UC 729-6 cells . The antibody reacted with carcinomas of the cervix (CaSki, HeLa), prostate (PC-3), breast (ZR-76-1), colon (COLO-205) and melanoma (G-361) but was negative for normal fibroblasts (WI-38 and MRC-9), T. lymphocytes and peripheral blood lymphocytes.
Application Notes
In therapy and diagnosis, both in vitro and in vivo.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
RPMI 1640
with 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose and 1500 mg/L sodium bicarbonate

, supplemented with 10% FBS.
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Target


Introduction
Cervical cancer is a malignant neoplasm arising from cells originating in the cervix uteri. One of the most common symptoms of cervical cancer is abnormal vaginal bleeding, but in some cases there may be no obvious symptoms until the cancer has progressed to an advanced stage.[1] Treatment usually consists of surgery (including local excision) in early stages, and chemotherapy and/or radiotherapy in more advanced stages of the disease.

Citations


Have you cited CSC-H0811 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !

Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * Please input "diagnostics" as verification code.

Online Inquiry

Order Info: Human Anti-Human Cervical Carcinoma Hybridoma [DMO I22.5]

Online Inquiry
  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!
  15% off your first purchase

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket