GeneralInformation
Immunogen
LPS activated DBA/2J mouse B cells
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Cell Line Description
Animals were immunized with LPS activated DBA/2J mouse B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the B7-2 (GL1) antigen present on activated mouse B cells.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Target
Synonyms
CD86; CD86 antigen; T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2;
Citations
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Brimnes, MK; Hansen, BE; et al. Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells. PLOS ONE 9:-(2014).
Faldyna, M; Samankova, P; et al. Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 119:56-62(2007).
COA
Certificate of Analysis
