Recombinant fusion protein of human CTCFL (NP_001255970.1).
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500-1:2000
General Notes
This product (Catalog No. DPABH-29173) is a rabbit-derived polyclonal antibody raised against human CTCFL. This antibody exhibits cross-reactivity with CTCFL from mouse and rat. It has been affinity purified by Protein A. CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. DPABH-29173 is suitable for use in Western blot (WB) (1:500–1:2,000) application. DPABH-29173 is supplied as a liquid formulation in PBS with 0.02% sodium azide,50% glycerol,pH7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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