Anti-CIT polyclonal antibody (DPAB-DC362)

Mouse Anti-Human CIT (aa 1928-2027) polyclonal antibody for WB, ELISA Datasheet

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Specifications


Host Species
Mouse
Species Reactivity
Human
Immunogen
CIT (NP_009105, 1928 a.a. ~ 2027 a.a) partial recombinant protein with GST tag. The sequence is TYNEHITKRVASSPAPPEGPSHPREPSTPHRYREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV
Conjugate
Unconjugated

Target


Alternative Names
CIT; citron rho-interacting serine/threonine kinase; CRIK; STK21; citron Rho-interacting kinase; serine/threonine kinase 21
Entrez Gene ID
UniProt ID

Product Background


Gene summary
CIT (Citron Rho-Interacting Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with CIT include schizophrenia. Among its related pathways are Signaling by GPCR and Signaling by Rho GTPases. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDC42BPA. This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants.
Antigen Description
This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants. Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2. Citron Rho-interacting kinase is an enzyme that in humans is encoded by the CIT gene. As previously mentioned, citron-K was believed to act in cytokinesis. Its depletion impairs maintenance of the midbody and its overexpression in HeLa cells rendered host cells multinucleated. Cytokinesis failure of Citron-K-depleted cells occurred after full ingression of the cleavage furrow, at the abscission stage. Microtubule disassembly was not seen in any of Citron-K depleted cells with cytokinesis failure. The dominant mode of failure was the inability of daughter cells, which are connected with a shorter intercellular bridge, to separate well. As the midbody microtubules were displaced from the center toward either of the two daughter cells, the two cells fused again with the microtubules absorbed into that daughter cell. To sum the process, Citron-K is important to keep proper structure of the midbody which holds the intercellular bridge microtubules between the two daughter cells and is thus required for successful transition from constriction to abscission. In molecular terms, citron-K depletion impaired the accumulation of 3 key proteins: Rho, Anillin and septins (specifically septin 6 and 7) in the intercellular bridge in mid–late telophase, which in previous stages early to mid-telophase was found to co-localize with them. This will lead our discussion of the interactions of Citron-K protein. The function about CIT antigen include ATP binding; PDZ domain binding; Rho GTPase binding; SH3 domain binding.
Pathway
G13 Signaling Pathway.

Citations


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References


Kobayashi, K; Chiba, K; et al. Identification of cytochrome p450 isoforms involved in citalopram N-demethylation by human liver microsomes. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 280:927-933(1997).
Copeland, A; Younes, A; et al. BRENTUXIMAB VEDOTIN Anti-CD30 Antibody-Drug Conjugate Oncolytic. DRUGS OF THE FUTURE 35:797-801(2010).

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